Kidney regenerative medicine

Humphreys lab

Towards Kidney Precision Medicines for Patients

single cell omics

We use single cell technologies to drive precision medicine

kidney organoids

We generate kidney organoids to model development & disease.

regenerative med

We develop treatments for human kidney diseases.

skill focus

high resolution microscopy

We use high resolution confocal microscopy to visualize expression of proteins by immunofluorescence, and mRNA by RNAscope. We also utilize three dimensional light sheet fluorescence microscopy to visualize branching of nephron-like structures in kidney organoids differentiated from human pluripotent stem cells.

★★★★★ 5/5

published work

selected research

research projects

single cell omics

A majority of the lab applies single nucleus RNA-seq and snATAC-seq to mouse and human kidney and lung. We have generated over 1.5M single cell libraries, and perform 100% of the bioinformatic analyses within the lab. See recent papers here, here and here. Our single cell data visualization tool Kidney Integrative Transcriptomics (K.I.T.) had 8,000 unique pageviews in 2019.

research projects

kidney organoids

Kidney organoids differentiated from pluripotent cells represent a powerful technology for modeling human development and disease. We refine and improve differentiation protocols, and characterize the organoids using single cell omics and high resolution 3D fluorescence microscopy. This paper was named Cell Stem Cell Best of 2018.