The Humphreys Laboratory

EXPERTISE

We use cutting-edge techniques to discover cures for kidney disease

mouse models

We culture mouse embryonic stem cells and perform gene targeting by homologous recombination. Our experience with genome modification allows us to generate unique models to interrogate disease models with precision.

Gene Editing

Crispr-Cas9 based gene editing offers genome editing with unparalleled specificity and speed. All trainees learn this technology which we apply in cell lines and stem cells.

Stem Cell Biology

The ability to generate patient-specific Pluripotent stem cells gives hope that we can grow replacement kidneys ‘in a dish’ that can someday be used in transplatation. This has become a major focus in the lab.

Human Translation

In order to validate discoveries in mouse, we have generated a substantial human kidney biobank. Current efforts focus on defining transcriptional profiles in individual kidney cell types from human kidney biopsies.

Watch the Lab Grow

The Lab

Our mission: To develop new cures for human kidney disease
Working together to cure kidney disease

Over 100,000 people per year develop kidney failure in the US and most are treated with dialysis therapy. While life-saving, this procedure is costly, inconvenient and accelerates heart disease.

An average dialysis patient has a 1 in 5 chance of dying every year. Paying for dialysis costs 7 percent of the entire Medicare budget, even though these patients make up only 1 percent of the Medicare population.

In The Humphreys Lab we are developing new and innovative treatments to help patients with kidney disease. We are using human stem cells to generate kidney organoids in a dish, with a goal of one day transplanting them into patients with kidney failure. We also study the kidney’s ability to regenerate itself so that we can harness this ability for therapeutic uses.

  • Genetic mouse models

    We have created 5 transgenic and 3 knockin mouse lines with 6 more in production

  • Crispr-Cas9

    We are using gene editing to create novel KO and KI iPS and mouse ES lines

  • Gene Profiling

    We use RNA-Seq to expression profile kidney cell types

  • Human kidney biobank

    Validation of novel therapeutic targets identified in mouse in human tissue

Our Team

Humphreys Lab Members at Washington University in St. Louis
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Benjamin D. Humphreys MD, PhD

Principal Investigator
Dr. Humphreys is the Chromalloy Associate Professor of Medicine and Chief of Nephrology at Washington University School of Medicine in St. Louis. His laboratory studies acute and chronic kidney disease.
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monica Chang-panesso MD

Postdoctoral Fellow
I received my MD from Texas Tech University in 2009 and completed nephrology fellowship at the Brigham and Women’s Hospital/ Massachusetts General Hospital in 2015. I joined the Humphreys Lab in July 2013 and focus on aging and acute kidney injury.
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Lucy Fan

Lab Manager
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Flavia Gomes-machado PhD

Postdoctoral Fellow
I received my Msc and PhD from University of Sao Paulo, Brazil in 2008 and 2012, respectively, for my studies in chronic kidney disease. I joined Humphreys Lab in 2013 and focus on mechanisms of kidney aging and the role of pericytes in acute and chronic kidney injuries.
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Yoichiro Ikeda MD, PhD

Postdoctoral Fellow
I received an MD degree from the University of Tokyo in 2002 and a PhD in nephrology in 2010. I joined the Humphreys Lab in 2013. I study stromal cells transformation to myofibroblasts using genetic approaches.
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Shiyo Ikeda MD, PhD

Research Associate
​​I received my M.D. in 2004 from the University of Tokyo and a Ph.D. in nephrology in 2012 there. I am engaged in the research on molecular mechanisms of acute kidney injury and repair.
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Farid Kadyrov, BS

Research Technician
I graduated from the University of Illinois in 2016 with a degree in Molecular and Cellular biology. I joined the lab in June 2016 and study acute kidney injury.
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Aileen Lu

Senior Research Technician
Pluripotent stem cell expert
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Eoghainín Ó hAinmhire PhD

Postdoctoral Fellow
I received my Ph.D from the University of Illinois at Chicago (2015) for my work in drug discovery for ovarian cancer. My focus is on resident renal mesenchymal stem cells and their role in both kidney repair and fibrosis.
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Ayano Miyagi MSc

Research Technician I
I received an MSc from the University of Tokyo (the graduate school for frontier sciences) in 2012. I joined the Humphreys lab in July 2015 and working on design and execution of CRISPR/ Cas9-based experiments with Aileen.
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Zhao Sun PhD

Postdoctoral Fellow
Zhao received his Ph.D. (May, 2016) from University of Iowa for his studies in craniofacial development. After graduation, he joined the Humphreys Lab and is working on kidney fibrosis using stem cell approaches.
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Kohei Uchimura PhD

I received my M.D. (2004) and Ph.D. (2012) at Kumamoto University. I joined the Humphreys Lab in August, 2015 and my research focuses on the identification of novel biomarkers of kidney fibrosis.
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Haojia Wu PhD

Postdoctoral Fellow
I completed my PhD studies in th Renal Division from The University of Hong Kong in 2014. I joined the Humphreys Lab in September, 2015, and I currently work on the molecular profiling of individual cell types in mouse and human kidney.

Recent Work

Published

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Lab News

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