We use cutting-edge techniques to discover cures for kidney disease
We culture mouse embryonic stem cells and perform gene targeting by using Crispr/Cas9 and HDR. Our experience with genome modification allows us to generate unique models to interrogate disease models with precision.
Crispr-Cas9 based gene editing offers genome editing with unparalleled specificity and speed. All trainees learn this technology which we apply in cell lines and stem cells.
Stem Cell Biology
The ability to generate patient-specific pluripotent stem cells gives hope that we can grow replacement kidneys ‘in a dish’ that can someday be used in transplantation. Understanding the potential of kidney organoids is a major focus.
We are leveraging single cell RNA-seq approaches including DropSeq, sNucDropSeq, InDrops and FACS-Seq to generate transcriptional atlases of kidney organoids and adult kidney in health and during the course of disease.
Watch the Lab Grow
Our mission: To develop new cures for human kidney disease
An average dialysis patient has a 1 in 5 chance of dying every year. Paying for dialysis costs 7 percent of the entire Medicare budget, even though these patients make up only 1 percent of the Medicare population.
In The Humphreys Lab we are developing new and innovative treatments to help patients with kidney disease. We are using human stem cells to generate kidney organoids in a dish, with a goal of one day transplanting them into patients with kidney failure. We also study the kidney’s ability to regenerate itself so that we can harness this ability for therapeutic uses.
Humphreys Lab Members at Washington University in St. Louis
Benjamin D. Humphreys MD, PhD
monica Chang-panesso MD
Assistant Professor of Medicine
Jeff Lai MD
Yoshiharu Muto, MD
Yuhei Kirita ,MD
Sai Mukund Ramakrishnan
Research Technician I
Zhao Sun PhD
Kohei Uchimura PhD
Haojia Wu PhD
Cell Stem Cell
J Am Soc Nephrol
J Clin Invest